《植物生理学报》 2011, 47(12): 1195-1200

H₂O₂介导H₂S诱导的拟南芥气孔关闭

青岛农业大学生命科学学院, 山东省高等学校植物生物技术重点实验室, 山东青岛266109

青岛农业大学生命科学学院, 山东省高等学校植物生物技术重点实验室, 山东青岛266109

通信作者：；E-mail: liuxin6080@yahoo.com.cn；Tel: 0532-88030224

摘　要：

以拟南芥(Arabidopsis thaliana)为材料, 研究了过氧化氢(H2O2)在硫化氢(H2S)调控气孔运动信号转导中的作用。结果 表明, 光下H2S的供体硫氢化钠(NaHS)能够诱导拟南芥气孔关闭; 且能够显著提高叶片和保卫细胞胞质H2O2含量; H2O2的清 除剂AsA和H2O2合成酶的抑制剂可不同程度地抑制NaHS诱导的拟南芥气孔关闭及叶片和保卫细胞胞质H2O2水平的升高; NaHS对AtrbohD、AtrbohF、Atpao2和Atpao4突变体气孔关闭、叶片和保卫细胞胞质H2O2水平升高的诱导作用要明显的小 于野生型, 但对AtPAO2和AtPAO4过表达株系叶片和保卫细胞H2O2水平的升高较野生型显著。据此推测, 来源于NADPH氧 化酶、细胞壁过氧化物酶和多胺氧化酶途径的H2O2参与H2S诱导的拟南芥气孔关闭。

关键词：硫化氢; 过氧化氢; 气孔关闭; 拟南芥

收稿：2011-08-24   修定：2011-10-09

资助：国家自然基金(30970228和31170237)、山东省自然基金 (ZR2010CM024)和植物生理学与生物化学国家重点实验 室开放课题(SKLPPBKF11001)。

H₂O₂Involvement in H₂S-Induced Stomatal Closure of Arabidopsis thaliana L.

YE Qing, HOU Zhi-Hui, LIU Jing, LIU Rui-Qing, LIU Xin*

College of Life Sciences, Qingdao Agricultural University, Key Lab of Plant Biotechnology in Universities of Shandong Province, Qingdao, Shandong 266109, China

Corresponding author: LIU Xin; E-mail: liuxin6080@yahoo.com.cn; Tel: 0532-88030224

Abstract:

Through pharmacological combined with spectrophotography and confocal laser scanning microscopy to study the effects of hydrogen peroxide (H₂O₂) in hydrogen sulphide (H₂S)-regulated guard cell movement of Arabidopsis thaliana. The results showed that NaHS (a donor of H₂S) caused stomatal closure in the light, and NaHS was able to induce H₂O₂ generation in guard cell cytoplasm and leaves of Arabidopsis thaliana obviously. The H₂O₂ scavenger and H₂O₂ synthesis inhibitors all prevented H₂S-induced stomatal closure and the level of H₂O₂ raised in guard cell and leaves. The effects of NaHS were more significant in AtrbohD, AtrbohF, Atpao2 and Atpao4 mutants than in wild-type, while in AtPAO2, AtPAO4 overexpression Arabidopsis were less effective. These results showed that H₂O₂ derived from NADPH oxidase, cell wall peroxidase and polyamine oxidase is involved in the signal transduction pathway of H₂S-induced stomatal closure in Arabidopsis thaliana.

Key words: hydrogen sulphide; hydrogen peroxide; stomatal closure; Arabidopsis thaliana